What is bacterioscopy and what is it for?

Bacteriosis is a diagnostic technique that allows to quickly and easily identify the occurrence of infections, because thanks to specific coloring techniques, it is possible to visualize the bacterial structures under the microscope.

This test can be performed with any biological material, and the physician must indicate which material should be collected and analyzed, and the result indicates whether or not the presence of bacteria has been verified, as well as the amount and characteristics observed.

  • Bacterioscopy is a diagnostic test that can be performed with any biological material and can be used to quickly identify bacterial infections:.

Additionally, bacterioscopy can be used to aid in the diagnosis of bacterial meningitis, diseases of the respiratory tract and gastrointestinal tract, and can be performed by biopsy or material from the anal area.

Thus, bacterioscopy is a laboratory technique that can be used in clinical practice to diagnose diseases caused by bacteria, indicating the characteristics of the agent causing the disease and, thus, allowing the doctor to start treatment even earlier. lab identification, which may take about 1 week.

The bacteriocopic examination is carried out in the laboratory and the material collected from the patient is analyzed under a microscope to look for the absence or presence of bacteria, as well as their characteristics.

Preparation for the exam depends on the material to be collected and analyzed. In the case of vaginal material, it is not recommended that the woman be cleaned 2 hours before the test and does not have sex in the last 24 hours, while in the case of taking nail or skin material, for example, it is recommended not to pass enamel, creams or substances on the skin before the test.

In the case of a vaginal discharge sample, for example, the swab that was used to collect the sample is passed in circular motions on a sheet, which must be identified with the patient’s initials and then stained with Gramme. For example, which is the material collected mainly to verify the presence of bacteria responsible for tuberculosis, the color used in bacteriosis is that of Ziehl-neelsen, which is more specific for this type of microorganism.

Usually, when the presence of bacteria is verified, the laboratory proceeds to the identification of the microorganism and the antibiogram, giving a more complete result.

Gram coloration is a simple and fast coloring technique that allows bacteria to be differentiated according to their characteristics, allowing bacteria to be differentiated into positive or negative according to their color, allowing them to be seen under the microscope.

This staining method uses two main dyes, one blue and one pink, which may or may not color the bacteria. Bacteria colored in blue are called gram-positive, while bacteria that are pink are called gram-negative. From this classification, it is possible for the doctor to start preventive treatment even before having the identification of the microorganism. Understand how the Gram stain is done and what it is for.

The purpose of the bacteriosis result is to indicate whether or not there are microorganisms, characteristics and quantity, in addition to the material that has been analyzed.

The result is said to be negative when microorganisms are not observed and positive when viewing microorganisms. The result is usually indicated by crosses ( ), where 1 indicates that 1 to 10 bacteria were observed in 100 fields, which may be a sign of an initial infection, for example, and 6 represents the presence of more than 1000 bacteria. by observed field, representing a more chronic infection or bacterial resistance, for example, indicating that treatment is not effective.

In addition, the report reported the staining used, which can be Gram or Ziehl-neelsen, for example, in addition to the characteristics of the micro-organism, such as form and arrangement, either in clusters or chains, for example.

Normally, when the result is positive, the laboratory identifies the microorganism and the antibiogram, indicating which antibiotic is the most recommended to treat infection by a certain bacterium.

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