Electrophoresis is a laboratory technique used to separate acid molecules from their size and electrical charge so that they can diagnose diseases, verify protein expression, or identify microorganisms.
Electrophoresis is a simple and inexpensive procedure, used as a routine technique in laboratories and research projects, depending on the purpose of electrophoresis, other problems and tests may be necessary to arrive at certain diagnoses.
- Electrophoresis can be performed for a variety of purposes.
- Both in research projects and diagnostics.
- As it is a simple and economical technique.
- In this way.
- Electrophoresis can be performed to:.
Depending on the purpose of electrophoresis, more tests may be needed for your doctor to complete the diagnosis.
Electrophoresis requires a gel, which can be polyacrylamide or agarosa, depending on the lens, the electrophoresis buffer and bowl, the molecular weight marker and fluorescent dye, as well as UV or LED light equipment, also known as a transluminator.
After gel preparation, a specific object called paint should be placed to make a cavity in the gel and let it sit, when the gel is listed, simply apply the substances to the cavities. should be placed in one of the wells, a positive control, which is a substance known for what it is; negative control, which guarantees the validity of the reaction, and the things to be analyzed. All songs must be mixed with a fluorescent dye, so that it is possible to visualize the tapes in the transiluminator.
The gel with the samples should be placed in the electrophoresis vessel, which contains the specific buffer solution, then adheres to the device to generate an electric current and therefore differentiates the potential, as is important for particle separation based on its load and the time of the electrophoretic stroke varied according to the purpose of the procedure and could last up to 1 hour.
After a while, it is possible to visualize the result of the electropholetic cycle using the transilluminator. When the gel is placed under UV or LED light, it is possible to see the band pattern: most of the molecule, the lower its migration, being more over the cavity; so much so that the more people who add to the molecule, the greater the migration potential.
For the reaction to be validated, the positive control tapes must be displayed and nothing is seen in the negative witness, but otherwise it indicates that the contamination is concentrated, thus repeating the whole process.
Electrophoresis can be performed for different purposes and, depending on its purpose, various types of gels can be used, the most common being polyacrylamide and agar soda.
Electrophoresis to identify microorganisms is the most common in research laboratories, however, for fine diagnosis, electrophoresis can be used to identify haematological diseases and diseases that progress with an increase in number. protein, with the main types of electrophoresis being:
Hemoglobin electrophoresis is a laboratory technique used to identify different types of hemoglobin circulating in the blood, and it is possible to identify the presence of diseases related to hemoglobin synthesis. The type of hemoglobin is identified by specific pH electrophoresis, ideally between 8. 0 and 9. 0, determining a band pattern that can be compared to the normal pattern, thus identifying the presence of abnormal hemoglobins.
What is done: Hemoglobin electrophoresis is performed with the aim of studying and diagnosing diseases related to hemoglobin synthesis, such as sickle cell disease and hemoglobin C disease, as well as being useful in differentiating thalassemia, learning to interpret hemoglobin electrophoresis.
Protein electrophoresis is a doctor’s request to evaluate the amount of protein circulating in your blood to identify diseases, this test is done from a blood sample, which is centrifuged to obtain plasma, which is the part of the blood that is made up, among other substances, of proteins.
An electrophoresis lamp might show a band model and then a graph showing the corner of each protein fraction, which is critical for diagnosis.
For the record, protein electrophoresis allows the physician to diagnose certain conditions, such as multiple myeloma, dehydration, cirrhosis, inflammation, liver disease, pancreatitis, lupus, and hypertension, based on the banding pattern and graph presented in the review report.
We know how to interpret the result of protein electrophoresis.